Enzyme electrode for free cholesterol
نویسندگان
چکیده
منابع مشابه
Enzyme electrode probes.
An electrical transducer for urea is described. The excellent stability of various types of urease enzyme electrodes developed in the author's laboratory is due to understanding the parameters which affect immobilized enzyme stability. Preliminary experiments have shown that a liquid layer of urease trapped in a double cellophane layer over a cation electrode may produce a useful enzyme electro...
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In this report, a new cholesterol sensor is developed based on a vertically aligned CNT electrode with two-step electrochemical polymerized enzyme immobilization. Vertically aligned CNTs are selectively grown on a 1 mm(2) window of gold coated SiO(2)/Si substrate by thermal chemical vapor deposition (CVD) with gravity effect and water-assisted etching. CNTs are then simultaneously functionalize...
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An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...
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In this study, we demonstrated a simple, rapid and inexpensive fabrication method to develop a novel gold nanobouquet structure fabricated indium tin oxide (GNB/ITO) electrode based on electrochemical deposition of gold ions onto ITO substrate. The morphology of the fabricated electrode surface was characterized by scanning electron microscopy (SEM) to confirm the GNB formation. Enzyme-free det...
متن کاملIntestinal cholesterol esterase: intracellular enzyme
The location of cholesterol esterase in rabbit intestine was re-evaluated. In three different experiments that were designed to eliminate contaminating mucus and pancreatic enzymes from the lumen of the small intestine, it was observed that the activities of cholesterol esterase and amylase in intestinal cytosol and whole homogenate decreased in parallel fashion. After the mucus was carefully w...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1977
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260190711